food and nutrition


Ascorbic

Question: Why is DCPIP a good indicator for finding the concentration of ascorbic acid? Please help, I am doing coursework on the concentration of vitamin C (ascorbic acid) in tablets and need to know why DCPIP is a good indicator. I know it has a sharp colour change but why is a stronger reducing agent not used? Please Help!!

Answer: DCPIP can also be used as an indicator for Vitamin C. If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ascorbic acid. DCPIP (blue) + H+ ----------> DCPIPH (pink) DCPIPH (pink) + VitC ----------> DCPIPH2 (colorless) C6H8O6 + C12H7NCl2O2 ----------> C6H6O6 + C12H9NCl2O2 In a titration, when all the ascorbic acid in the solution has been used up, there will not be any electrons available to reduce the DCPIPH and the solution will remain pink due to the DCPIPH. The end point is a pink color that persists for 10 seconds or more. http://en.wikipedia.org/wiki/DCPIP


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